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BlotGlyco® Glycan Purification and Labeling Kit

Product Description

BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture

  • BlotGlyco® is a sample preparation kit for glycan analysis of biological samples.
  • Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on.
  • BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities.
  • BlotGlyco® kit is a quite useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.

Application

Sample Preparation for Glycan Analysis

GlycoBeads for Glycan chart.png

Our sample preperation for glycan analysis is a streamlined process, optimized for screening N-glycan in large sample batches during cell line selection, upstream and downstream process development.

A streamlined process, optimized for screening N-glycans in large sample batches during cell line selection, upstream and downstream process development.

  • Glycosylation analysis of biologics (for research, screening, quality control and production process monitoring)
  • Glycosylation analysis of cells, especially for the differentiation researches of stem cells
  • Combination of glycomics and proteomics (glycans can be analyzed from electrohoresis bands)
  • Glycan biomarker discovery

Product Description

BlotGlyco® Beads: Polymer beads are densely covered with hydrazide groups to facilitate glycan capture

  • BlotGlyco® is a sample preparation kit for glycan analysis of biological samples.
  • Using this kit will purify and label glycans from the crude samples such as glycoprotein, serum, cell, organ, plant tissue and so on. The label is selectable depending on detection methods including HPLC, LC-MS, MALDI-TOF MS and so on.
  • BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities.
  • BlotGlyco® kit is a quite useful tool for isolating glycans from the variety of biological samples, selectively and comprehensively.

Features

  • Purification and labeling are completed in about 5 hours using just one spin tube
  • No special equipment is necessary except a heating block and a small desktop centrifuge. No column chromatography is necessary.
  • Glycans can be purified and labeled from various crude biological samples. Conventional fluorescent labeling reagents (2AB, PA) are applicable. Kit contains a unique labeling reagent for the high sensitive MALDI-TOF MS measurement.
  • By using a 96 well filter plate and a vacuum manifold, as many as 96 samples are processed simultaneously.

Automating Glycan Sample Prep

BlotGlyco-Glycan Purification and Labeling Kit

Experiments

Biomarker discovery

  • N-glycan of human serum: using 5μL serum
    blotglyco-exp-biomarker

Glycosylation analysis of Biologics

  • N-glycan of antibody: using 10μg IgG
    blotglyco-exp-biologics

Additional information

Weight N/A
Dimensions N/A

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BlotGlyco® Glycan Purification and Labeling Kit

$450.00$7,200.00

Cat. No.ItemPolymer BeadsAowrReaction TubeCleanup ColumnPriceQuantity
BS-45403ZBlotGlyco 1010 Assaysfor 10 Assays1010$500.00
BS-45404ZBlotGlyco 5050 Assaysfor 50 Assays5050$2,250.00
BS-45405ZBlotGlyco 100100 Assaysfor 100 Assays100100$4,000.00
BS-45406ZBlotGlyco 100A100 Assaysfor 100 AssaysNo Reaction TubesNo Cleanup Columns$3,760.00
BS-45407ZBlotGlyco 100B100 AssaysNo aoWR100100$3,840.00
BS-45408ZBlotGlyco 100C100 AssaysNo aoWRNo Reaction TubesNo Cleanup Columns$3,600.00
BS-45409ZBlotGlyco 100D100 AssaysNo aoWRNo Reaction Tubes100$3,760.00
BS-45410ZBlotGlyco 200C200 AssaysNo aoWRNo Reaction TubesNo Cleanup Columns$7,200.00
BS-45411ZBlotGlyco 100E100 AssaysNo aoWR100No Cleanup Columns$3,680.00
BS-45412ZBlotGlyco 200E200 AssaysNo aoWR200No Cleanup Columns$7,200.00
BS-45413ZBlotGlyco 96 plate B96 AssaysNo aoWR1 Filterplate1 Cleanup Plate$3,840.00
BS-45414ZBlotGlyco 10B10 AssaysNo aoWR1010$450.00
BS-45415ZBlotGlyco 50B50 AssaysNo aoWR5050$2,025.00
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