Low Cell Attachment Plate PrimeSurface®

Product Description

Ultra-Low Attachment 3D Cell Culture Plate PrimeSurface®

The inner wall of the PrimeSurface® cell culture ware is pre-coated with our unique ultra hydrophilic polymer. This prevents a cell from adhering to the plastic surface which induces a spontaneous cell spheroid formation in equal size and shape.
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Publications List

Cell Culture

For Stem Cell Research:

Differentiation induction of ES, iPS and mesenchymal stem cells from embryoid body formation.

For Drug Screening Research & Development:

An ideal product for drug response studies. Three dimension spheroid models are more physiologically relevant than two dimension monolayer model.

For Tissue engineering and Regenerative medicine:

Regenova is a novel robotic system that facilitates the fabrication of three- dimensional cellular structures by placing cellular spheroids in fine needle arrays according to pre-designed 3D data. Followings are examples of such fabrications using S-BIO’s PrimeSurface® 96U plate and Bio 3D Printer, Regenova (Cyfuse Biomedical K.K.).; neural 3D tissue and 3D tissue with mesenchymal stem cells.

 

Neural 3D tissue
Cell sources: hiPSC derived neural progenitor cells
Quantity of cells:4 X 104 cells/well
Culture Medium:For neural cells
Maturation duration on Well Plate:2 days
3D structure of printed 3D tissue:printed with 3 X 3 X 2
Quantity of spheroids used per 3D tissue:18 spheroids
Maturation duration after 3D printing:Remove needles 9 days after 3D print

Spheroid
(formed in PrimeSurface® 96U plate)
3D printing with “Regenova” 3D tissue after removal of needles

 

 

3D tissue with mesenchymal stem cells
Cell sources: human adipose tissue derived mesenchymal stem cells (hADSC)
Quantity of cells 5×103 cells/well
Culture Medium:For MSC
Maturation duration on Well Plate:2 days
3D structure of printed 3D tissue:Circle shape with 48 spheroids x 10 layers
Quantity of spheroids used per 3D tissue:480 spheroids
Maturation duration after 3D printing:Remove needles 6 days after 3D print

(Spheroid formed in PrimeSurface® 96U plate) 3D printing with “Regenova” (above) 3D printing with “Regenova” (side view) 3D tissue after removal of needles

For more information, please visit Cyfuse Biomedical K.K.’s website.
https://www.cyfusebio.com/en/product/

Features

  • The various well bottom shapes provide the optimum well shape for your cell properties
    Well bottom shapes
  • Uniform single spheroid formation in one well
  • No influence caused by plate material
  • Stable, non-cytotoxic and cell non-adhesion surface
  • Easy handling, compatible with liquid robotic system
  • Sterilized individual packaging

PrimeSurface Flyer for Drug Screening

PrimeSurface flyer for Stem cell research

One-Pot Intracellular ATP Assay with PrimeSurface® White Plate

 

Experiments

Time-lapse imaging of human iPS cells spheroid (embryoid body) formation

CULTURE CONDITIONS:

  • Culture plate:PrimeSurface MS-9096V
  • Cells:hiPS cell (201B7: Takahashi K et al. Cell, 2007 Nov 30;131(5):861-72, iPS Academia Japan, Inc.)
  • Seeding density:9,000 cells/well
  • Culture medium:DMEM/F12 + 20% (v/v) KSR + 1% (v/v) NEAA + L-Glutamine (2mM) + Β-Mercaptethanol (80μM) + Y-27632(30μM)
  • Culture environment:5%CO2, 37°C

Application example of hES cells differentiation using PrimeSurface®

Self-formation of neural retina tissue from the aggregate of human ES cells by using PrimeSurface MS-9096V

PrimeSurface-hES

Culture plate: PrimeSurface MS-9096V

Cell type: Human ES cells (KhES-1 strain)

Seeding density: 9,000 cells/well

Culture medium: GMEM+KSR+NEAA+2ME+ 20uM Y-27632

Culture environment: 5%CO2, 37°C

Data resource

Picture a-c: Division of Human Stem Cell Technology RIKEN Center for Developmental Biology

Reference

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs; NakanoT, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y; Cell Stem Cell, 10 (6), 771-785 (2012)

Evaluation example of anticancer drug efficacy by using PrimeSurface®

PrimeSurface-anticancer
Cell Type: MCF-7 (Human breast cancer cell)

Anticancer drug: 5-Fluorouracil (5FU)

Data resource

Nishio Lab., Department of Genome Biology, Kinki University School of Medicine

Please check the supplemental data for more detail example of application.

Specification of Ultra-Low Attachment 3D Cell Culture Plate PrimeSurface®

Cat. No Product Description Growth Area Volume Qty/Pk Qty/Cs
MS-9035XZ PrimeSurface Dish 35 φ 35 φ × 14(H)mm 9 cm2 5 50
MS-9060XZ PrimeSurface Dish 60 φ 60 φ × 15(H)mm 21 cm2 10 100
MS-9090XZ PrimeSurface Dish 90 φ 90 φ × 20(H)mm 57 cm2 10 50
MS-9024XZ PrimeSurface Plate 24F 24wells, Flat 1.8 cm2 3.4mL/well 1 10

Remark
Radiation sterilized
Preserved at room temperature, Product life: 2 years after production

Cat. No Product Wells Bottom Volume Qty/Pk Qty/Cs
MS-9384UZ PrimeSurface 384U 384 U bottom 0.1mL 1 20
MS-9384WZ PrimeSurface 384U White Plate 384 U bottom 0.1mL 1 20
MS-9096VZ PrimeSurface 96V 96 V bottom 0.3mL 1 20
MS-9096MZ PrimeSurface 96M 96 Spindle bottom 0.2mL 1 20
MS-9096UZ PrimeSurface 96U 96 U bottom 0.3mL 1 20
MS-9096WZ PrimeSurface 96U White Plate 96 U bottom 0.3mL 1 20

Remark
Radiation sterilized
Preserved at room temperature, Product life: 2 years after production

Publications List

Primesurface® Stem Cell Research List

< Retinal Research >

  1. KUWAHARA, Atsushi, et al. Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. Nature Communications, 2015, 6.: 1-15[ MS-9096V ]
  2. TANAKA, T., et al. Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells. Sci Rep, 2015, 5. 8344. [ MS-9096V ]
  3. NAKANO, Tokushige, et al. Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs. Cell Stem Cell, 2012, 10. 6: 771-785. [ MS-9096V ]
  4. KAINI, Ramesh, et al. Xeno-free 3D retinal differentiation of human induced-pluripotent stem cells. Investigative ophthalmology & visual science, 2014, 55. 13: 1369-1369. [ MS-9096V ]
  5. EIRAKU, Mototsugu and SASAI, Yoshiki Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues. NATURE PROTOCOLS, 2012, 7. 1: 69-79. [ MS-9096U ]

< Neuroscience Research >

  1. 1. MUGURUMA, Keiko, et al. Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells. Cell Reports, 2015, 10:537-550 [ MS-9096V ]
  2. BAMBA, Y., et al. Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochem Biophys Res Commun, 2014, 447. 4: 683-688. [ MS-9096V ]
  3. MINAMINO, Yuki, et al. Isolation and Propagation of Neural Crest Stem Cells from Mouse Embryonic Stem Cells via Cranial Neurospheres. Stem cells and development, 2014, 24.2: 172-181 [ MS-9096 U, M or V ]
  4. KADOSHIMA, T., et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110. 50: 20284-20289. [ MS-9096V ]
  5. OGAWA, Yasuhiro, et al. Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease. PLoS ONE, 2013, 8. 1: e55856. [ MS-9096 U, M or V ]
  6. GOMI, Masanori, et al. Functional recovery of the murine brain ischemia model using human induced pluripotent stem cell-derived telencephalic progenitors. Brain research, 2012, 1459. 52-60. [ MS-9035X ]
  7. NASU, Makoto, et al. Robust formation and maintenance of continuous stratified cortical neuroepithelium by laminin-containing matrix in mouse ES cell culture. PLoS ONE, 2012, 7. 12: e53024. [ MS-9096 U, M or V ]
  8. DANJO, T., et al. Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory trea®ent with extrinsic signals. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011, 31. 5: 1919-1933. [ MS-9096 U ]
  9. KANEMURA, Yonehiro Development of cell-processing systems for human stem cells (neural stem cells, mesenchymal stem cells, and iPS cells) for regenerative medicine. The Keio journal of medicine, 2010, 59. 2: 35-45. [ MS-9096 U ]

 

< Cardiomyocytes and Heart Research >

  1. NOGUCHI, Ryo, et al. Development of a Three-Dimensional Prevascularized Scaffold-Free Contractile Cardiac Patch for Treating Heart Disease. The Journal of Heart and Lung Transplantation, 2015, [ MS-9096 ]
  2. TAKASHIMA, Yasuhiro, et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 2014, 158. 6: 1254-1269. [ MS-9096V ]
  3. OTSUJI, Tomomi G, et al. Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes. PLoS ONE, 2012, 7. 9: e45010. [ MS-9035X ]
  4. SATOSHI, Yasuda, et al. AW551984: a novel regulator of cardiomyogenesis in pluripotent embryonic cells. Biochemical Journal, 2011, 437. 2: 345-355. [ MS-9096U ]
  5. YASUDA, S., et al. A novel regulator of cardiomyogenesis in pluripotent embryonic cells. The Biochemical journal, 2011, 437. 2: 345-355. [ MS-9096U ]
  6. OTSUJI, Tomomi G, et al. Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs. Stem cell research, 2010, 4. 3: 201-213. [ MS-9096U ]
  7. YAMAUCHI, Kaori, et al. Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs. Genes to Cells, 2010, 15. 12: 1216-1227. [ MS-9035X, MS-9060X or MS-9090X ]

< Hepatocyte Research >

  1. YANAGIDA, Ayaka, et al. Liver maturation deficiency in p57 Kip2-/-mice occurs in a hepatocytic p57 Kip2 expression-independent manner. Developmental biology, 2015, [ MS-9096U ]
  2. ISHII, Takamichi (2012). Differentiation of Human Embryonic Stem Cells into Functional Hepatocyte-Like Cells (Method). Stem Cells and Cancer Stem Cells, Volume 2, Springer: 43-49. [ MS-9096 U, M or V ]
  3. ISHII, Takamichi et al. (2012). Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers. Human Embryonic and Induced Pluripotent Stem Cells, Springer: 397-403. [ MS-9096 U, M or V ]
  4. ISHII, Takamichi, et al. In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers. Cell and tissue research, 2010, 339. 3: 505-512. [ MS-9096U ]

 

< Bone and Cartliage Research >

  1. MURATA, Daiki, et al. A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells. Journal of orthopaedic surgery and research, 2015, 10. 1: 1-12. [ MS-9096 U, M or V ]
  2. SATO, Kazutoshi, et al. Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology-Animal, 2015, 51. 5: 515-529. 1. [ MS-9096 U, M or V ]
  3. FUJIMOTO, Mai, et al. Establishment of a novel model of chondrogenesis using murine embryonic stem cells carrying fibrodysplasia ossificans progressiva-associated mutant ALK2. Biochemical and Biophysical Research Communications, 2014, 455. 3: 347-352. 1. [ MS-9096 U, M or V ]
  4. ISHIHARA, Kohei, et al. Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells. J Orthop Surg Res, 2014, 9. 1: 98. 1. [ MS-9096 U ]

< Dental Research >

  1. OZEKI, Nobuaki, et al. Differentiation of Human Skeletal Muscle Stem Cells into Odontoblasts Is Dependent on Induction of α1 Integrin Expression. Journal of Biological Chemistry, 2014, 289. 20: 14380-14391. [ MS-9035X, MS-9060X or MS-9090X ]
  2. YAMAMOTO, Mioko, et al. Three-dimensional spheroid culture promotes odonto/osteoblastic differentiation of dental pulp cells. Archives of oral biology, 2014, 59. 3: 310-317. [ MS-9096 U, M or V ]

< Somniferous Tubule Research >

  1. YOKONISHI, T., et al. In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation. Biol Reprod, 2013, 89. (1):15: 1–6. [ MS-9096V ]

< Generation of iPS Cell >

  1. OHNISHI, Hiroe, et al. Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Cellular Reprogramming (Formerly” Cloning and Stem Cells”), 2015, 17. 4: 268-274.
  2. OHNISHI, Hiroe, et al. A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow-and adipose tissue-derived mesenchymal stem cells. Journal of tissue engineering and regenerative medicine, 2012, 6. 4: 261-271. 1. [ MS-9035X ]
  3. AOKI, T., et al. Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC. Tissue engineering. Part A, 2010, 16. 7: 2197-2206. [ MS-9035X ]
  4. ODA, Y., et al. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. J Biol Chem, 2010, 285. 38: 29270-29278. [ MS-9035X, MS-9060X or MS-9090X ]

< Others >

  1. IMAI, Hiroyuki, et al. Tetraploid Embryonic Stem Cells Maintain Pluripotency and Differentiation Potency into Three Germ Layers. PLoS ONE, 2015, 10. 6: e0130585. [ MS-9096U ]
  2. MITSUI, Kaoru, et al. Conditionally replicating adenovirus prevents pluripotent stem cell–derived teratoma by specifically eliminating undifferentiated cells. Molecular Therapy. Methods & Clinical Development, 2015, 2. 15026. [ MS-9096U, MS-9096M or MS-9096V]
  3. ZHOU, Yuanshu, et al. Metabolic suppression during mesodermal differentiation of embryonic stem cells identified by single-cell comprehensive gene expression analysis. Molecular BioSystems, 2015, 11. 9: 2560-2567. [ MS-9096U ]
  4. TAKASHIMA, Yasuhiro, et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 2014, 158. 6: 1254-1269. [MS-9096V ]
  5. KIMURA, Kenichi, et al. The Role of CCL5 in the Ability of Adipose Tissue-Derived Mesenchymal Stem Cells to Support Repair of Ischemic Regions.Stem cells and development, 2013, 23. 5: 488-501. [MS-9096U ]
  6. SHIMOTO, Takeshi, et al. (2013). Bio Rapid Prototyping Project: Development of Spheroid Formation System for Regenerative Medicine. Information Technology Convergence, Springer: 855-862. [ MS-9096 U, M or V ]
  7. KOIDE, Naoshi, et al. Establishment and optimal culture conditions of microRNA-induced pluripotent stem cells generated from HEK293 cells via transfection of microRNA-302s expression vector. Nagoya journal of medical science, 2012, 74. 1-2: 157-165. [ MS-9096 U, M or V ]
  8. MARKS, H., et al. The transcriptional and epigenomic foundations of ground state pluripotency. Cell, 2012, 149. 3: 590-604. [MS-9096U ]
  9. OHNISHI, Hiroe, et al. (2012). Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods). Human Embryonic and Induced Pluripotent Stem Cells, Springer: 173-190. [ MS-9035X, MS-9060X or MS-9090X ]
  10. SAKAI, Yusuke, et al. Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips. Journal of bioscience and bioengineering, 2011, 111. 1: 85-91. [ MS-9096U ]
  11. TAKAYAMA, Yuzo, et al. Toward the Precise Control of Cell Differentiation Processes by Using Micro and Soft Lithography. 2011, [ MS-9096 U, M or V ]
  12. TAKAYAMA, Yuzo, et al. Simultaneous induction of calcium transients in embryoid bodies using microfabricated electrode substrates.Journal of bioscience and bioengineering, 2011, 112. 6: 624-629. [ MS-9096U ]
  13. TANASIJEVIC, Borko and RASMUSSEN, Theodore P X chromosome inactivation and differentiation occur readily in ES cells doubly-deficient for macroH2A1 and macroH2A2. PLoS ONE, 2011, 6. 6: e21512. [ MS-9096U ]
  14. KATAOKA, Ken, et al. Internalization of REIC/Dkk-3 protein by induced pluripotent stem cell-derived embryoid bodies and extra-embryonic tissues. Int J Mol Med, 2010, 26. 6: 853-859. [ MS-9096U ]
  15. TAKAYAMA, Yuzo, et al. (2009). Ensemble stimulation of embryoid bodies using microfabricated ITO substrates. Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE, IEEE. [ MS-9096U ]
  16. TAKAYAMA, Yuzo, et al. Ensemble Stimulation of Embryoid Bodies using Substrate-Embedded Electrodes. IEEJ Transactions on Electrical and Electronic Engineering, 2009, 4. 6: 734-735. [ MS-9096U ]

< ESTEmbryonic Stem Cell Test >

  1. SUZUKI, N., et al. Evaluation of novel high-throughput embryonic stem cell tests with new molecular markers for screening embryotoxic chemicals in vitro. Toxicological sciences : an official journal of the Society of Toxicology, 2011, 124. 2: 460-471. [ MS-9096W ]

Note: Some references refer to the product name as “Cell-Tight ”. Cell Tight was renamed to PrimeSurface®.

[PrimeSurface® Cancer Research Publication List]

  1. ROTEM, Asaf, et al. Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation. Proceedings of the National Academy of Sciences, 2015,
  2. MISU, Masayasu, et al. Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells. Biochemical and Biophysical Research Communications, 2015, 463. 4: 618-623.
  3. NAKANISHI, Yoshito, et al. Mechanism of oncogenic signal activation by the novel fusion kinase FGFR3–BAIAP2L1. Molecular cancer therapeutics, 2015, 14. 3: 704-712.
  4. NOZAWA-SUZUKI, Noriko, et al. The inhibitory effect of hypoxic cytotoxin on the expansion of cancer stem cells in ovarian cancer. Biochemical and Biophysical Research Communications, 2015, 457. 4: 706-711.
  5. YONESAKA, K, et al. Anti-HER3 monoclonal antibody patritumab sensitizes refractory non-small cell lung cancer to the epidermal growth factor receptor inhibitor erlotinib. Oncogene, 2015,
  6. YOSHIDA, Ryohei, et al. EGFR tyrosine kinase inhibitors combined with cytotoxic drugs for treatment of NSCLC with EGFR gene mutations: Efficacy and mechanisms. Cancer research, 2015, 75. 15 Supplement: 3501-3501.
  7. KODAMA, Tatsushi, et al. A Novel Mechanism of EML4-ALK Rearrangement Mediated by Chromothripsis in a Patient-Derived Cell Line. Journal of Thoracic Oncology, 2014,
  8. KODAMA, Tatsushi, et al. Alectinib shows potent antitumor activity against RET-rearranged non–small cell lung cancer. Molecular cancer therapeutics, 2014, 13. 12: 2910-2918.
  9. MIKHAIL, Andrew S, et al. Image-Based Analysis of the Size-and Time-Dependent Penetration of Polymeric Micelles in Multicellular Tumor Spheroids and Tumor Xenografts. International journal of pharmaceutics, 2014, 464. 1–2: 168–177.
  10. MORI, Masamichi, et al. The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice. Molecular cancer therapeutics, 2014, 13. 2: 329-340.
  11. OHNISHI, Ken, et al. Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International journal of oncology, 2014, 45. 2: 581-586.
  12. SHIMOZATO, O., et al. Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation. Oncogene (Nature), 2014, 1-12.
  13. AKIMOTO, Miho, et al. An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells. World J Med Genet, 2013, 27. 3(4): 41-45.
  14. BRESLIN, Susan and O’DRISCOLL, Lorraine Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today, 2013, 18. 5: 240-249.
  15. GOUDARZI, Houman, et al. Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma. Biomedical Research, 2013, 34. 1: 13-21.
  16. ISHII, Genichiro, et al. Morphophenotype of floating colonies derived from a single cancer cell has a critical impact on tumor-forming activity. Pathology International, 2013, 63. 1: 29-36.
  17. KATO, Takuma, et al. Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells. PLoS ONE, 2013, 8. 12: e82876.
  18. KESSEL, Sarah, et al. (2013). Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method. Molecular biology of the cell, AMER SOC CELL BIOLOGY 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA.
  19. MIKHAIL, Andrew S, et al. Multicellular Tumor Spheroids for Evaluation of Cytotoxicity and Tumor Growth Inhibitory Effects of Nanomedicines In Vitro: A Comparison of Docetaxel-Loaded Block Copolymer Micelles and Taxotere®. PLoS ONE, 2013, 8. 4: e62630.
  20. SATO, Shuji, et al. Identification of the Cancer Cell Proliferation and Survival Functions of proHB-EGF by Using an Anti-HB-EGF Antibody. PLoS ONE, 2013, 8. 1: e54509.
  21. KONISHI, Hiroaki, et al. PEGylated liposome IHL-305 markedly improved the survival of ovarian cancer peritoneal metastasis in mouse. BMC Cancer, 2012, 12. 1: 462.
  22. MASUDA, Taisuke, et al. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomedical microdevices, 2012, 14. 6: 1085-1093.
  23. NISHIMURA, S., et al. MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. PLoS ONE, 2012, 7. 6: e38618.
  24. OYANAGI, Jun, et al. Epithelial-mesenchymal transition stimulates human cancer cells to extend microtubule-based invasive protrusions and suppresses cell growth in collagen gel. PLoS ONE, 2012, 7. 12: e53209.
  25. UNO, Makiko, et al. Identification of physiologically active substances as novel ligands for MRGPRD. BioMed Research International, 2012, 2012. 1-9.
  26. KOSHIKAWA, Naohiko, et al. Proteolytic activation of heparin-binding EGF-like growth factor by membrane-type matrix metalloproteinase-1 in ovarian carcinoma cells. Cancer science, 2011, 102. 1: 111-116.
  27. KUNITA, Akiko, et al. Podoplanin is regulated by AP-1 and promotes platelet aggregation and cell migration in osteosarcoma. The American journal of pathology, 2011, 179. 2: 1041-1049.
  28. SAKAMOTO, Hiroshi, et al. CH5424802, a selective ALK inhibitor capable of blocking the resistant gatekeeper mutant. Cancer cell, 2011, 19. 5: 679-690.
  29. SAKUMA, Yuji, et al. WZ4002, a third-generation EGFR inhibitor, can overcome anoikis resistance in EGFR-mutant lung adenocarcinomas more efficiently than Src inhibitors. Laboratory Investigation, 2011, 92. 3: 371-383.
  30. SHIMIZU, Yutaka, et al. Dienogest, a synthetic progestin, inhibits prostaglandin E2 production and aromatase expression by human endometrial epithelial cells in a spheroid culture system. Steroids, 2011, 76. 1: 60-67.
  31. KOGASHIWA, Yasunao, et al. Docetaxel suppresses invasiveness of head and neck cancer cells in vitro. Cancer science, 2010, 101. 6: 1382-1386.
  32. MATSUYAMA, Masahiro, et al. Reduced CD73 expression and its association with altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases. Oncology Letters, 2010, 1. 3: 431-436.
  33. YAMAGUCHI, Shigeru, et al. Novel Photodynamic Therapy Using Water-dispersed TiO2–Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro. Photochemistry and photobiology, 2010, 86. 4: 964-971.
  34. HAN, M., et al. Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles. Mol Ther, 2009, 17. 8: 1404-1410.
  35. WATANABE, Y, et al. A novel translational approach for human malignant pleural mesothelioma: heparanase-assisted dual virotherapy. Oncogene (Nature), 2009, 29. 8: 1145-1154.
  36. HAN, M., et al. Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies. J Control Release, 2007, 121. 1-2: 38-48.
  37. KUNITA, Akiko, et al. The platelet aggregation-inducing factor aggrus/podoplanin promotes pulmonary metastasis. The American journal of pathology, 2007, 170. 4: 1337-1347.

[PrimeSurface® Other application Publication List]

  1. ICHIOKA, Masayuki, et al. Dienogest, a synthetic progestin, down-regulates expression of CYP19A1 and inflammatory and neuroangiogenesis factors through progesterone receptor isoforms A and B in endometriotic cells. The Journal of steroid biochemistry and molecular biology, 2015, 147. 103-110.
  2. MORI, Taisuke, et al. Dienogest reduces HSD17β1 expression and activity in endometriosis. Journal of Endocrinology, 2015, 225. 2: 69-76.
  3. PARSONS, Matthew W, et al. Dectin-2 Regulates the Effector Phase of House Dust Mite–Elicited Pulmonary Inflammation Independently from Its Role in Sensitization. The Journal of Immunology, 2014, 192. 4: 1361-1371.
  4. BRESLIN, Susan and O’DRISCOLL, Lorraine Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today, 2013, 18. 5: 240-249.
  5. BARRETT, Nora A, et al. Cysteinyl leukotriene 2 receptor on dendritic cells negatively regulates ligand-dependent allergic pulmonary inflammation. The Journal of Immunology, 2012, 189. 9: 4556-4565.
  6. MASUDA, Taisuke, et al. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomedical microdevices, 2012, 14. 6: 1085-1093.
  7. SOMA, Tsutomu, et al. Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation. Experimental dermatology, 2012, 21. 4: 307-309.
  8. YAMANAKA, Kaoruko, et al. Dienogest inhibits aromatase and cyclooxygenase-2 expression and prostaglandin E2 production in human endometriotic stromal cells in spheroid culture. Fertil Steril, 2012, 97. 2: 477-482.
  9. BRENNAN, Patrick J, et al. Invariant natural killer T cells recognize lipid self antigen induced by microbial danger signals. Nature immunology, 2011, 12. 12: 1202-1211.
  10. YOSHIIKE, Yuka and KITAOKA, Takuya Tailoring hybrid glyco-nanolayers composed of chitohexaose and cellohexaose for cell culture applications. Journal of Materials Chemistry, 2011, 21. 30: 11150-11158.
  11. MAEKAWA, Akiko, et al. GPR17 regulates immune pulmonary inflammation induced by house dust mites. The Journal of Immunology, 2010, 185. 3: 1846-1854.
  12. TAMADA, Atsushi, et al. Autonomous right-screw rotation of growth cone filopodia drives neurite turning. The Journal of Cell Biology, 2010, 188. 3: 429-441.
  13. IJIMA, Hiroyuki, et al. Composition of culture medium is more important than co-culture with hepatic non-parenchymal cells in albumin production activity of primary rat hepatocytes, and the effect was enhanced by hepatocytes spheroid culture in collagen gel. BIOCHEMICAL ENGINEERING JOURNAL, 2009, 45. 3: 226-231.
  14. ITO, Michiko and TAGUCHI, Tetsushi Enhanced insulin secretion of physically crosslinked pancreatic β-cells by using a poly (ethylene glycol) derivative with oleyl groups. Acta Biomater, 2009, 5. 8: 2945-2952.
  15. KATAOKA, M, et al. Detection of biomarker for periodontal disease using a microchip.2008,

Low Cell Attachment Plate PrimeSurface®

$90.00$625.00

FormatGrowth AreaVolumeQty per CasePriceSKUQuantity
35mm dish9 sq. cm-50$90.00#MS-9035XZ
60mm dish21 sq. cm-100$400.00#MS-9060XZ
90mm dish57 sq. cm-50$375.00#MS-9090XZ
Plate 24F1.8 sq. cm3.4mL/well10$120.00#MS-9024XZ
96U platena0.3mL20$300.00#MS-9096UZ
96U White platena0.3mL20$400.00#MS-9096WZ

Out of stock

96M platena0.2mL20$400.00#MS-9096MZ
96V platena0.3mL20$500.00#MS-9096VZ
384U platena0.1mL20$500.00#MS-9384UZ
384U White platena0.1mL20$625.00#MS-9384WZ
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