EZGlyco® O-Glycan Prep Kit

STREAMLINED, RAPID and RELIABLE HPLC and LC-MS-Ready SAMPLE PREPARATION of O-GLYCANS

EZGlyco® O-Glycan Prep Kit provides a streamlined, rapid and reliable O-glycan sample preparation. The complete kit includes all reagents to release, enrich, label and purify O-glycans for HPLC and LC-MS analysis. The kit adopts a unique O-glycan releasing method using a high reactive amine and an organic superbase, which yields high recovery and less degradation (peeling) of released O-glycans. Applications include characterization of O-glycosylation of recombinant glycoproteins in upstream R&D, quality control and process control. It is also suited for O-glycan biomarker discovery in biological samples such as mucins, serum and exosome, and O-glycan analysis of functional foods such as dairy products.
O-Glycan Prep Kit Workflow

Customer Testimony

“S-BIO’s EZGlyco® O-Glycan Prep Kit allows for relatively quick and reliable O-glycan analysis in support of ongoing BioPharma projects.”

Jason Koch, Senior Analytical Scientist at a global animal health pharmaceutical company

“I used EZGlyco® O-Glycan Prep Kit for O-glycan sample prep of fusion protein and found it to be highly sensitive in UPLC analysis.   The chromatogram was comparable to hydrazinolysis method with the added advantage of good repeatability.  Typically, it takes me 1 day or more for O-glycan sample preparation using traditional kits, but I was pleasantly surprised with the quick 5 hr method with this kit.”

Senior Research Scientist, FivePrime Therapeutics

O-Glycan Sample Prep in 5 hours

O-Glycan sample preparation is completed in 5 hours with its unique O-glycan releasing reagents. Thus, HPLC/LC-MS analysis is achieved on the same day.

High Recovery

A combination of the unique O-glycan releasing reagent and the new O-Glycan Capturing Beads enables high recovery of released O-glycans from various glycoproteins.

Minimize “Peeling”

Glycan releasing reagents minimize decomposition of O-glycans (peeling), resulting in O-glycan characterization with higher accuracy.

Easy and Safe Operation

With a simple protocol to follow, O-glycan sample preparation is completed without any special laboratory equipment and toxic reagents.

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EZGlyco O-Glycan Prep Kit

EZGlyco® O-Glycan
Prep Kit Components

  • Protocol
  • Glycan Release Reagent A
  • Glycan Release Reagent B
  • Glycan Capturing Beads
  • Filter Column
  • 2-Aminobezamide
  • Reducing Reagent
  • Cleanup Column

Product & Ordering Information

Product Name: EZGlyco® O-Glycan Prep Kit
Catalog Number:
BS-41601Z
Content: For 10 Tests

Request pricing information by submitting the form below or contact us at:
info.s-bio@s-bio.com | 603.425.9697

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