Culturing cells in three-dimensions (3D) has become a well established approach as it is more representative of the in vivo environment than traditional two-dimensional (2D) cultures. Allowing cells to interact with each other in a spheroid creates a micro-environment which mimics in vivo tissue and is a better model for examining the effect of drugs [...]
OZAWA, Masayuki (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports. 5: 1-16.
Madin-Darby canine kidney cell: MDCK T23 cells, anchorage independence growth of tumor cells, Immunoprecipitation and immunoblotting Flow cytometry Anoikis assay Fluorescence microscopy WST-1 cell pr liferation assay Soft agar colony assay Gene expression microarray and data analysis, Phase contrast micrograph Fluorescence microscopy, anchorage independence growth of tumor cells E-cadherin N-cadherin YAP/TAZ signaling, Doxorubicin (DOX), PrimeSurface
High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry.
Glioblastoma: U87MG, high-throughput screening by Image cytometry, Spheroid and Cell Viability Spheroid Size and Adherent Cell Confluence Measurement, Fluorescent Staining Celigo Image Cytometer, 3D tumor spheroid, spheroid size, spheroid viability, glioblastoma, high-throughput screening, image cytometry, Celigo, 17-AAG paclitaxel TMZ doxorubicin(DOX), MS-9384U
S-Bio Launches EZGlyco mAB Kit with 2-AB for N Glycan Sample Preparation from IgG in cell culture for HPLC analysis
Hudson, NH, September 7, 2016 - S-BIO1 has launched today EZGlycoTM mAb-N Kit with 2-AB, a breakthrough new product that enables scientists to analyze N -glycans in IgG and Fc-fusion proteins from culture supernatant samples in a rapid, simplified and streamlined single tube operation. The kit is a valuable tool supporting customers in glycoprotein characterization [...]
(Co-located with Antibody Engineering and Therapeutics China) August 12-13, 2015 | Grand Hyatt Hotel, Shanghai, China http://www.ibclifesciences.com/BPIChina/overview.xml
S-BIO launched a new product, BlotGlyco® O-Glycan Preparation Kit, for O-Glycan analysis. The operations are very safe and simple over hydrazinolysis owing to using a mild ammonium salt for the releasing reaction. For details, click here.