Background
【Fig.3】 3D culture of DPCs shows high expression of HIF1A, and HIF-1α regulates trichogenic genes in DPC spheroids. (For details, please refer to the paper)
The hair cycle refers to the phenomenon of the anagen (active growth phase of hair), catagen (transitional stage and hair growth slows down), telogen (resting phase for new hair to grow) and new anagen of hair follicles. In androgenetic alopecia (AGA) patients, it is considered that the anagen phase gradually shortens so that the hair becomes shorter and thinner.
Dermal papilla cells (DPCs) plays important roles in the development and growth process of hair follicles, but how they contribute to the mechanism of hair regeneration, particularly in terms of alterations in AGA patients, is not yet fully understood.
Research Achievements
HIF-1α (hypoxia-inducible factor-1α) is a transcription factor that is activated when cells enter a state of hypoxia. In this study, HIF1A was highly expressed in healthy scalp tissue, and from in vitro experiments using 3D culture of DPCs mimicking in vivo environment, the author’s group successfully demonstrated that HIF-1α is suppressed in scalp tissues of androgenetic alopecia patients and potentially associated with hair follicle development. Maintaining DPCs with high HIF-1α levels could be applicable to prepare DPCs with enhanced hair inductivity for hair implantation. From a therapeutic perspective, targeting HIF-1α can be a potential strategy for recovering hair loss.
Use of PrimeSurface in this Study
3D cell cultures are used in many studies as it maintains the hair inductivity.
PrimeSurface® 96U plate was used for DPC spheroid formation and HIF-1α expression was compared between spheroid (3D culture) and monolayer culture. Regulation of HIF-1α was also investigated.
Cells: DPCs (PromoCell, Heidelberg, Germany) , si-Con and si-HIF-1α treated DPCs
Seeding density: 1✕104 cells/well
Medium：Follicle dermal papilla cell growth medium (PromoCell, Heidelberg, Germany)
【Fig.3】 3D culture of DPCs shows high expression of HIF1A, and HIF-1α regulates trichogenic genes in DPC spheroids. (For details, please refer to the paper)
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