Why White Plates Are The Best Choice For Luminescence Assays
Chemiluminescent assays often outperform analogous fluorescent assays due to its’ low background offering a high signal to noise ratio. White plates reflect light and maximize light output signal, thus providing higher assay sensitivity.
PrimeSurface® cultureware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly of spheroid formation. The plates are pre-coated with unique ultra hydrophilic polymer that enables spontaneous spheroid formation of uniform size and shape.
Spheroid tumor cells are typically generated using ULA plates and other 3D methods, however, transferring them for luminescence read-out to a white plate without damaging the spheroids can be challenging. Using PrimeSurface® 96 and 384 well ULA White plates, cancer spheroid cells can be formed and analyzed in the same multi-well plate for luminescence assays.
Streamlining the process reduces the experimental steps and time required for drug testing, furthermore prevents spheroid damage and provides a robust, high sensitivity assay for luminescent based high throughput screening.
Streamlined luminescence drug screening assay with
spheroid 3D culture cells using PrimeSurface® 384 well White plates