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PrimeSurface Spheroid Plates
S-Bio PrimeSurface Plates

PrimeSurface® 3D Culture Spheroid plates: Ultra-low Attachment (ULA) Plates

$103.00 – $718.00

IN-STOCK & READY TO SHIP!

Form Uniform Spheroids with Ultra-low Attachment PrimeSurface® Cell Culture Plate

PrimeSurface® cultureware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly of spheroid formation. The plates are pre-coated with unique ultra hydrophilic polymer that enables spontaneous spheroid formation of uniform size and shape.   The ULA plates have high optical clarity making them highly suitable for bright field imaging and confocal microscopy.  In addition to the widely used 96 well U bottom plate, 96 well plates are also available in V and M bottom, giving scientists a choice to form tighter spheroids that are needed for specific cell types.  For high throughput screening (HTS) needs,  384 well plates are available in clear and white.
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Product Description

Form Uniform Spheroids with Ultra-low Attachment PrimeSurface® Cell Culture Plate

PrimeSurface® cultureware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly of spheroid formation. The plates are pre-coated with unique ultra hydrophilic polymer that enables spontaneous spheroid formation of uniform size and shape.   The ULA plates have high optical clarity making them highly suitable for bright field imaging and confocal microscopy.  In addition to the widely used 96 well U bottom plate, 96 well plates are also available in V and M bottom, giving scientist a choice to form tighter spheroids that are needed for specific cell types.  For high throughput screening (HTS) needs,  384 well plates are available in clear and white.

“We’ve used the S-BIO PrimeSurface spheroid 96 V bottom plates in formation of brain organoids.  PrimeSurface (MS9096VZ) plates were an essential component for aggregation of stem cells into embryoid bodies to initiate organoid development.”
Rami Tshuva , Weizmann Institute of Science

Cell Culture and Assays

Stem Cell Research:

Differentiation induction of ES, iPS and mesenchymal stem cells from embryoid body formation.

Drug Screening Research & Development:

Three dimension spheroid models are more physiologically relevant than two dimension monolayer model.

Grow uniform spheroids and Screen for drug response in 96 and 384 well plates.

384 white plates are suitable for Chemiluminescence assays screening.

Compatible with  brightfield and fluorescence Imaging systems:

3D Spheroid Applications using ultra low attachment (ULA) plates:  

Hepatic 3D Spheroid Models for the detection and study of compounds with cholestatic liabilities  

Growth Inhibition of 3D Tumor Spheres

Fluorescent Image Analysis–Cell Division in SpheroidsFluorescent Image Analysis–Cell Division in Spheroids

Fluorescent Image Analysis–Live Dead Cell Assay of SpheroidsFluorescent Image Analysis–Live Dead Cell Assay of Spheroids

Evaluation of Drug Efficacy–The Response of Co-Cultured Spheroids to DrugsEvaluation of Drug Efficacy–The Response of Co-Cultured Spheroids to Drugs

Analysis of Anticancer Drugs-Targeting the Interior of SpheroidsAnalysis of Anticancer Drugs-Targeting the Interior of Spheroid

Evaluation of Drug Efficacy–Quantitative Evaluation of Spheroid SizeEvaluation of Drug Efficacy–Quantitative Evaluation of Spheroid Size

Evaluation of Drug Efficacy–The Response of Spheroids to a DrugEvaluation of Drug Efficacy–The Response of Spheroids to a Drug

Cell Counting–Measuring the Number of Cells in SpheroidsCell Counting–Measuring the Number of Cells in Spheroids

Tissue engineering and Regenerative medicine:

Regenova is a novel robotic system that facilitates the fabrication of three- dimensional cellular structures by placing cellular spheroids in fine needle arrays according to pre-designed 3D data. Followings are examples of such fabrications using S-BIO’s PrimeSurface® 96U plate and Bio 3D Printer, Regenova (Cyfuse Biomedical K.K.).; neural 3D tissue and 3D tissue with mesenchymal stem cells.

 

Neural 3D tissue
Cell sources： hiPSC derived neural progenitor cells
Quantity of cells：4 X 104 cells/well
Culture Medium：For neural cells
Maturation duration on Well Plate：2 days
3D structure of printed 3D tissue：printed with 3 X 3 X 2
Quantity of spheroids used per 3D tissue：18 spheroids
Maturation duration after 3D printing：Remove needles 9 days after 3D print

Spheroid
(formed in PrimeSurface® 96U plate)		3D printing with “Regenova”3D tissue after removal of needles

 

 

3D tissue with mesenchymal stem cells
Cell sources： human adipose tissue derived mesenchymal stem cells (hADSC)
Quantity of cells 5×103 cells/well
Culture Medium：For MSC
Maturation duration on Well Plate：2 days
3D structure of printed 3D tissue：Circle shape with 48 spheroids x 10 layers
Quantity of spheroids used per 3D tissue：480 spheroids
Maturation duration after 3D printing：Remove needles 6 days after 3D print

(Spheroid formed in PrimeSurface® 96U plate)3D printing with “Regenova” (above)3D printing with “Regenova” (side view)3D tissue after removal of needles

For more information, please visit Cyfuse Biomedical K.K.’s website.
https://www.cyfusebio.com/en/product/

Features

  • The various well bottom shapes of ULA plates provide options for optimum spheroid growth for different cell types.  Even though the U bottom  plates are most widely used, the V bottom and M (Spindle) bottom wells would be a preferred choice for some cell types, especially when developing tight spheroids
    Well bottom shapes
  • Uniform single spheroid formation in each well
  • Stable, non-cytotoxic and cell non-adhesion surface
  • Easy handling, compatible with liquid robotic system
  • Sterilized individual packaging

 

Experiments

Time-lapse imaging of human iPS cells spheroid (embryoid body) formation 

CULTURE CONDITIONS:

  • Culture plate：PrimeSurface MS-9096V
  • Cells：hiPS cell (201B7: Takahashi K et al. Cell, 2007 Nov 30;131(5):861-72, iPS Academia Japan, Inc.)
  • Seeding density：9,000 cells/well
  • Culture medium：DMEM/F12 + 20% (v/v) KSR + 1% (v/v) NEAA + L-Glutamine (2mM) + Β-Mercaptethanol (80μM) + Y-27632(30μM)
  • Culture environment：5%CO2, 37°C

Application example of hES cells differentiation using PrimeSurface®

Self-formation of neural retina tissue from the aggregate of human ES cells by using PrimeSurface MS-9096V

PrimeSurface-hES

Culture plate: PrimeSurface MS-9096V

Cell type: Human ES cells (KhES-1 strain)

Seeding density: 9,000 cells/well

Culture medium: GMEM+KSR+NEAA+2ME+ 20uM Y-27632

Culture environment: 5%CO2, 37°C

Data resource

Picture a-c: Division of Human Stem Cell Technology RIKEN Center for Developmental Biology

Reference

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs; NakanoT, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y; Cell Stem Cell, 10 (6), 771-785 (2012)

Evaluation example of anticancer drug efficacy by using PrimeSurface®

PrimeSurface-anticancer
Cell Type: MCF-7 (Human breast cancer cell)

Anticancer drug: 5-Fluorouracil (5FU)

Data resource

Nishio Lab., Department of Genome Biology, Kinki University School of Medicine

 

Specification of Ultra-Low Attachment 3D Cell Culture Plate PrimeSurface®

Cat. NoProductDescriptionGrowth AreaVolumeQty/PkQty/Cs
MS-9035XZPrimeSurface Dish 35 φ, Clear35 φ × 14(H)mm9 cm2–550
MS-9060XZPrimeSurface Dish 60 φ, Clear60 φ × 15(H)mm21 cm2–10100
MS-9090XZPrimeSurface Dish 90 φ, Clear90 φ × 20(H)mm57 cm2–1050
MS-9024XZPrimeSurface Plate 24F, Clear24wells, Flat1.8 cm23.4mL/well110

Remark
Radiation sterilized
Preserved at room temperature, Product life: 2 years after production

Cat. NoProductWellsBottomVolumeQty/PkQty/Cs
MS-9384UZPrimeSurface 384U, Clear384U bottom0.1mL120
MS-9384WZPrimeSurface 384U White Plate384U bottom0.1mL120
MS-9096VZPrimeSurface  96V, Clear96V bottom0.3mL120
MS-9096UZPrimeSurface 96U, Clear96U bottom0.3mL120
MS-9096WZPrimeSurface 96U White Plate96U bottom0.3mL120

Remark
Radiation sterilized
Preserved at room temperature, Product life: 2 years after production

Publications List

【Primesurface® Stem Cell Research List】

< Retinal Research >

  1. KUWAHARA, Atsushi, et al. Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. Nature Communications, 2015, 6.: 1-15[ MS-9096V ]
  2. TANAKA, T., et al. Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells. Sci Rep, 2015, 5. 8344. [ MS-9096V ]
  3. NAKANO, Tokushige, et al. Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs. Cell Stem Cell, 2012, 10. 6: 771-785. [ MS-9096V ]
  4. KAINI, Ramesh, et al. Xeno-free 3D retinal differentiation of human induced-pluripotent stem cells. Investigative ophthalmology & visual science, 2014, 55. 13: 1369-1369. [ MS-9096V ]
  5. EIRAKU, Mototsugu and SASAI, Yoshiki Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues. NATURE PROTOCOLS, 2012, 7. 1: 69-79. [ MS-9096U ]

< Neuroscience Research >

  1. 1. MUGURUMA, Keiko, et al. Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells. Cell Reports, 2015, 10:537-550 [ MS-9096V ]
  2. BAMBA, Y., et al. Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics. Biochem Biophys Res Commun, 2014, 447. 4: 683-688. [ MS-9096V ]
  3. MINAMINO, Yuki, et al. Isolation and Propagation of Neural Crest Stem Cells from Mouse Embryonic Stem Cells via Cranial Neurospheres. Stem cells and development, 2014, 24.2: 172-181 [ MS-9096 U, M or V ]
  4. KADOSHIMA, T., et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110. 50: 20284-20289. [ MS-9096V ]
  5. OGAWA, Yasuhiro, et al. Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease. PLoS ONE, 2013, 8. 1: e55856. [ MS-9096 U, M or V ]
  6. GOMI, Masanori, et al. Functional recovery of the murine brain ischemia model using human induced pluripotent stem cell-derived telencephalic progenitors. Brain research, 2012, 1459. 52-60. [ MS-9035X ]
  7. NASU, Makoto, et al. Robust formation and maintenance of continuous stratified cortical neuroepithelium by laminin-containing matrix in mouse ES cell culture. PLoS ONE, 2012, 7. 12: e53024. [ MS-9096 U, M or V ]
  8. DANJO, T., et al. Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory trea®ent with extrinsic signals. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011, 31. 5: 1919-1933. [ MS-9096 U ]
  9. KANEMURA, Yonehiro Development of cell-processing systems for human stem cells (neural stem cells, mesenchymal stem cells, and iPS cells) for regenerative medicine. The Keio journal of medicine, 2010, 59. 2: 35-45. [ MS-9096 U ]

 

< Cardiomyocytes and Heart Research >

  1. NOGUCHI, Ryo, et al. Development of a Three-Dimensional Prevascularized Scaffold-Free Contractile Cardiac Patch for Treating Heart Disease. The Journal of Heart and Lung Transplantation, 2015, [ MS-9096 ]
  2. TAKASHIMA, Yasuhiro, et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 2014, 158. 6: 1254-1269. [ MS-9096V ]
  3. OTSUJI, Tomomi G, et al. Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes. PLoS ONE, 2012, 7. 9: e45010. [ MS-9035X ]
  4. SATOSHI, Yasuda, et al. AW551984: a novel regulator of cardiomyogenesis in pluripotent embryonic cells. Biochemical Journal, 2011, 437. 2: 345-355. [ MS-9096U ]
  5. YASUDA, S., et al. A novel regulator of cardiomyogenesis in pluripotent embryonic cells. The Biochemical journal, 2011, 437. 2: 345-355. [ MS-9096U ]
  6. OTSUJI, Tomomi G, et al. Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs. Stem cell research, 2010, 4. 3: 201-213. [ MS-9096U ]
  7. YAMAUCHI, Kaori, et al. Cardiomyocytes develop from anterior primitive streak cells induced by β-catenin activation and the blockage of BMP signaling in hESCs. Genes to Cells, 2010, 15. 12: 1216-1227. [ MS-9035X, MS-9060X or MS-9090X ]

< Hepatocyte Research >

  1. YANAGIDA, Ayaka, et al. Liver maturation deficiency in p57 Kip2-/-mice occurs in a hepatocytic p57 Kip2 expression-independent manner. Developmental biology, 2015, [ MS-9096U ]
  2. ISHII, Takamichi (2012). Differentiation of Human Embryonic Stem Cells into Functional Hepatocyte-Like Cells (Method). Stem Cells and Cancer Stem Cells, Volume 2, Springer: 43-49. [ MS-9096 U, M or V ]
  3. ISHII, Takamichi et al. (2012). Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers. Human Embryonic and Induced Pluripotent Stem Cells, Springer: 397-403. [ MS-9096 U, M or V ]
  4. ISHII, Takamichi, et al. In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers. Cell and tissue research, 2010, 339. 3: 505-512. [ MS-9096U ]

< Toxicology >

  1. Ryuji Kato and Jack Uetrecht. Supernatant from Hepatocyte Cultures with Drugs That Cause Idiosyncratic Liver Injury Activates Macrophage Inflammasomes. Chem. Res. Toxicol. 2017,30,1327-1332 [MS-9096U]
  2. Alastair Mak*, Ryuji Kato†, Kyle Weston*, Anthony Hayes‡, and Jack Uetrecht*,et al.   An Impaired Immune Tolerance Animal Model Distinguishes the Potential of Troglitazone/Pioglitazone and  Tolcapone/Entacapone to Cause IDILI.  TOXICOLOGICAL SCIENCES, 2017, 1–9  [ MS-9096U ]

< Bone and Cartilage Research >

  1. MURATA, Daiki, et al. A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells. Journal of orthopaedic surgery and research, 2015, 10. 1: 1-12. [ MS-9096 U, M or V ]
  2. SATO, Kazutoshi, et al. Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells. In Vitro Cellular & Developmental Biology-Animal, 2015, 51. 5: 515-529. 1. [ MS-9096 U, M or V ]
  3. FUJIMOTO, Mai, et al. Establishment of a novel model of chondrogenesis using murine embryonic stem cells carrying fibrodysplasia ossificans progressiva-associated mutant ALK2. Biochemical and Biophysical Research Communications, 2014, 455. 3: 347-352. 1. [ MS-9096 U, M or V ]
  4. ISHIHARA, Kohei, et al. Simultaneous regeneration of full-thickness cartilage and subchondral bone defects in vivo using a three-dimensional scaffold-free autologous construct derived from high-density bone marrow-derived mesenchymal stem cells. J Orthop Surg Res, 2014, 9. 1: 98. 1. [ MS-9096 U ]

< Dental Research >

  1. OZEKI, Nobuaki, et al. Differentiation of Human Skeletal Muscle Stem Cells into Odontoblasts Is Dependent on Induction of α1 Integrin Expression. Journal of Biological Chemistry, 2014, 289. 20: 14380-14391. [ MS-9035X, MS-9060X or MS-9090X ]
  2. YAMAMOTO, Mioko, et al. Three-dimensional spheroid culture promotes odonto/osteoblastic differentiation of dental pulp cells. Archives of oral biology, 2014, 59. 3: 310-317. [ MS-9096 U, M or V ]

< Semniferous Tubule Research >

  1. YOKONISHI, T., et al. In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation. Biol Reprod, 2013, 89. (1):15: 1–6. [ MS-9096V ]

< Generation of iPS Cell >

  1. OHNISHI, Hiroe, et al. Generation of Xeroderma Pigmentosum-A Patient-Derived Induced Pluripotent Stem Cell Line for Use As Future Disease Model. Cellular Reprogramming (Formerly” Cloning and Stem Cells”), 2015, 17. 4: 268-274.
  2. OHNISHI, Hiroe, et al. A comparative study of induced pluripotent stem cells generated from frozen, stocked bone marrow-and adipose tissue-derived mesenchymal stem cells. Journal of tissue engineering and regenerative medicine, 2012, 6. 4: 261-271. 1. [ MS-9035X ]
  3. AOKI, T., et al. Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC. Tissue engineering. Part A, 2010, 16. 7: 2197-2206. [ MS-9035X ]
  4. ODA, Y., et al. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells. J Biol Chem, 2010, 285. 38: 29270-29278. [ MS-9035X, MS-9060X or MS-9090X ]

< Others >

  1. IMAI, Hiroyuki, et al. Tetraploid Embryonic Stem Cells Maintain Pluripotency and Differentiation Potency into Three Germ Layers. PLoS ONE, 2015, 10. 6: e0130585. [ MS-9096U ]
  2. MITSUI, Kaoru, et al. Conditionally replicating adenovirus prevents pluripotent stem cell–derived teratoma by specifically eliminating undifferentiated cells. Molecular Therapy. Methods & Clinical Development, 2015, 2. 15026. [ MS-9096U, MS-9096M or MS-9096V]
  3. ZHOU, Yuanshu, et al. Metabolic suppression during mesodermal differentiation of embryonic stem cells identified by single-cell comprehensive gene expression analysis. Molecular BioSystems, 2015, 11. 9: 2560-2567. [ MS-9096U ]
  4. TAKASHIMA, Yasuhiro, et al. Resetting transcription factor control circuitry toward ground-state pluripotency in human. Cell, 2014, 158. 6: 1254-1269. [MS-9096V ]
  5. KIMURA, Kenichi, et al. The Role of CCL5 in the Ability of Adipose Tissue-Derived Mesenchymal Stem Cells to Support Repair of Ischemic Regions.Stem cells and development, 2013, 23. 5: 488-501. [MS-9096U ]
  6. SHIMOTO, Takeshi, et al. (2013). Bio Rapid Prototyping Project: Development of Spheroid Formation System for Regenerative Medicine. Information Technology Convergence, Springer: 855-862. [ MS-9096 U, M or V ]
  7. KOIDE, Naoshi, et al. Establishment and optimal culture conditions of microRNA-induced pluripotent stem cells generated from HEK293 cells via transfection of microRNA-302s expression vector. Nagoya journal of medical science, 2012, 74. 1-2: 157-165. [ MS-9096 U, M or V ]
  8. MARKS, H., et al. The transcriptional and epigenomic foundations of ground state pluripotency. Cell, 2012, 149. 3: 590-604. [MS-9096U ]
  9. OHNISHI, Hiroe, et al. (2012). Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods). Human Embryonic and Induced Pluripotent Stem Cells, Springer: 173-190. [ MS-9035X, MS-9060X or MS-9090X ]
  10. SAKAI, Yusuke, et al. Embryoid body culture of mouse embryonic stem cells using microwell and micropatterned chips. Journal of bioscience and bioengineering, 2011, 111. 1: 85-91. [ MS-9096U ]
  11. TAKAYAMA, Yuzo, et al. Toward the Precise Control of Cell Differentiation Processes by Using Micro and Soft Lithography. 2011, [ MS-9096 U, M or V ]
  12. TAKAYAMA, Yuzo, et al. Simultaneous induction of calcium transients in embryoid bodies using microfabricated electrode substrates.Journal of bioscience and bioengineering, 2011, 112. 6: 624-629. [ MS-9096U ]
  13. TANASIJEVIC, Borko and RASMUSSEN, Theodore P X chromosome inactivation and differentiation occur readily in ES cells doubly-deficient for macroH2A1 and macroH2A2. PLoS ONE, 2011, 6. 6: e21512. [ MS-9096U ]
  14. KATAOKA, Ken, et al. Internalization of REIC/Dkk-3 protein by induced pluripotent stem cell-derived embryoid bodies and extra-embryonic tissues. Int J Mol Med, 2010, 26. 6: 853-859. [ MS-9096U ]
  15. TAKAYAMA, Yuzo, et al. (2009). Ensemble stimulation of embryoid bodies using microfabricated ITO substrates. Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE, IEEE. [ MS-9096U ]
  16. TAKAYAMA, Yuzo, et al. Ensemble Stimulation of Embryoid Bodies using Substrate-Embedded Electrodes. IEEJ Transactions on Electrical and Electronic Engineering, 2009, 4. 6: 734-735. [ MS-9096U ]

< EST（Embryonic Stem Cell Test） >

  1. SUZUKI, N., et al. Evaluation of novel high-throughput embryonic stem cell tests with new molecular markers for screening embryotoxic chemicals in vitro. Toxicological sciences : an official journal of the Society of Toxicology, 2011, 124. 2: 460-471. [ MS-9096W ]

Note: Some references refer to the product name as “Cell-Tight ”. Cell Tight was renamed to PrimeSurface®.

[PrimeSurface® Cancer Research Publication List]

  1. ROTEM, Asaf, et al. Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation. Proceedings of the National Academy of Sciences, 2015,
  2. MISU, Masayasu, et al. Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells. Biochemical and Biophysical Research Communications, 2015, 463. 4: 618-623.
  3. NAKANISHI, Yoshito, et al. Mechanism of oncogenic signal activation by the novel fusion kinase FGFR3–BAIAP2L1. Molecular cancer therapeutics, 2015, 14. 3: 704-712.
  4. NOZAWA-SUZUKI, Noriko, et al. The inhibitory effect of hypoxic cytotoxin on the expansion of cancer stem cells in ovarian cancer. Biochemical and Biophysical Research Communications, 2015, 457. 4: 706-711.
  5. YONESAKA, K, et al. Anti-HER3 monoclonal antibody patritumab sensitizes refractory non-small cell lung cancer to the epidermal growth factor receptor inhibitor erlotinib. Oncogene, 2015,
  6. YOSHIDA, Ryohei, et al. EGFR tyrosine kinase inhibitors combined with cytotoxic drugs for treatment of NSCLC with EGFR gene mutations: Efficacy and mechanisms. Cancer research, 2015, 75. 15 Supplement: 3501-3501.
  7. KODAMA, Tatsushi, et al. A Novel Mechanism of EML4-ALK Rearrangement Mediated by Chromothripsis in a Patient-Derived Cell Line. Journal of Thoracic Oncology, 2014,
  8. KODAMA, Tatsushi, et al. Alectinib shows potent antitumor activity against RET-rearranged non–small cell lung cancer. Molecular cancer therapeutics, 2014, 13. 12: 2910-2918.
  9. MIKHAIL, Andrew S, et al. Image-Based Analysis of the Size-and Time-Dependent Penetration of Polymeric Micelles in Multicellular Tumor Spheroids and Tumor Xenografts. International journal of pharmaceutics, 2014, 464. 1–2: 168–177.
  10. MORI, Masamichi, et al. The selective anaplastic lymphoma receptor tyrosine kinase inhibitor ASP3026 induces tumor regression and prolongs survival in non-small cell lung cancer model mice. Molecular cancer therapeutics, 2014, 13. 2: 329-340.
  11. OHNISHI, Ken, et al. Plastic induction of CD133AC133-positive cells in the microenvironment of glioblastoma spheroids. International journal of oncology, 2014, 45. 2: 581-586.
  12. SHIMOZATO, O., et al. Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation. Oncogene (Nature), 2014, 1-12.
  13. AKIMOTO, Miho, et al. An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells. World J Med Genet, 2013, 27. 3(4): 41-45.
  14. BRESLIN, Susan and O’DRISCOLL, Lorraine Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today, 2013, 18. 5: 240-249.
  15. GOUDARZI, Houman, et al. Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma. Biomedical Research, 2013, 34. 1: 13-21.
  16. ISHII, Genichiro, et al. Morphophenotype of floating colonies derived from a single cancer cell has a critical impact on tumor-forming activity. Pathology International, 2013, 63. 1: 29-36.
  17. KATO, Takuma, et al. Protein Transfection Study Using Multicellular Tumor Spheroids of Human Hepatoma Huh-7 Cells. PLoS ONE, 2013, 8. 12: e82876.
  18. KESSEL, Sarah, et al. (2013). Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method. Molecular biology of the cell, AMER SOC CELL BIOLOGY 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA.
  19. MIKHAIL, Andrew S, et al. Multicellular Tumor Spheroids for Evaluation of Cytotoxicity and Tumor Growth Inhibitory Effects of Nanomedicines In Vitro: A Comparison of Docetaxel-Loaded Block Copolymer Micelles and Taxotere®. PLoS ONE, 2013, 8. 4: e62630.
  20. SATO, Shuji, et al. Identification of the Cancer Cell Proliferation and Survival Functions of proHB-EGF by Using an Anti-HB-EGF Antibody. PLoS ONE, 2013, 8. 1: e54509.
  21. KONISHI, Hiroaki, et al. PEGylated liposome IHL-305 markedly improved the survival of ovarian cancer peritoneal metastasis in mouse. BMC Cancer, 2012, 12. 1: 462.
  22. MASUDA, Taisuke, et al. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomedical microdevices, 2012, 14. 6: 1085-1093.
  23. NISHIMURA, S., et al. MRGD, a MAS-related G-protein coupled receptor, promotes tumorigenisis and is highly expressed in lung cancer. PLoS ONE, 2012, 7. 6: e38618.
  24. OYANAGI, Jun, et al. Epithelial-mesenchymal transition stimulates human cancer cells to extend microtubule-based invasive protrusions and suppresses cell growth in collagen gel. PLoS ONE, 2012, 7. 12: e53209.
  25. UNO, Makiko, et al. Identification of physiologically active substances as novel ligands for MRGPRD. BioMed Research International, 2012, 2012. 1-9.
  26. KOSHIKAWA, Naohiko, et al. Proteolytic activation of heparin-binding EGF-like growth factor by membrane-type matrix metalloproteinase-1 in ovarian carcinoma cells. Cancer science, 2011, 102. 1: 111-116.
  27. KUNITA, Akiko, et al. Podoplanin is regulated by AP-1 and promotes platelet aggregation and cell migration in osteosarcoma. The American journal of pathology, 2011, 179. 2: 1041-1049.
  28. SAKAMOTO, Hiroshi, et al. CH5424802, a selective ALK inhibitor capable of blocking the resistant gatekeeper mutant. Cancer cell, 2011, 19. 5: 679-690.
  29. SAKUMA, Yuji, et al. WZ4002, a third-generation EGFR inhibitor, can overcome anoikis resistance in EGFR-mutant lung adenocarcinomas more efficiently than Src inhibitors. Laboratory Investigation, 2011, 92. 3: 371-383.
  30. SHIMIZU, Yutaka, et al. Dienogest, a synthetic progestin, inhibits prostaglandin E2 production and aromatase expression by human endometrial epithelial cells in a spheroid culture system. Steroids, 2011, 76. 1: 60-67.
  31. KOGASHIWA, Yasunao, et al. Docetaxel suppresses invasiveness of head and neck cancer cells in vitro. Cancer science, 2010, 101. 6: 1382-1386.
  32. MATSUYAMA, Masahiro, et al. Reduced CD73 expression and its association with altered purine nucleotide metabolism in colorectal cancer cells robustly causing liver metastases. Oncology Letters, 2010, 1. 3: 431-436.
  33. YAMAGUCHI, Shigeru, et al. Novel Photodynamic Therapy Using Water-dispersed TiO2–Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro. Photochemistry and photobiology, 2010, 86. 4: 964-971.
  34. HAN, M., et al. Enhanced percolation and gene expression in tumor hypoxia by PEGylated polyplex micelles. Mol Ther, 2009, 17. 8: 1404-1410.
  35. WATANABE, Y, et al. A novel translational approach for human malignant pleural mesothelioma: heparanase-assisted dual virotherapy. Oncogene (Nature), 2009, 29. 8: 1145-1154.
  36. HAN, M., et al. Transfection study using multicellular tumor spheroids for screening non-viral polymeric gene vectors with low cytotoxicity and high transfection efficiencies. J Control Release, 2007, 121. 1-2: 38-48.
  37. KUNITA, Akiko, et al. The platelet aggregation-inducing factor aggrus/podoplanin promotes pulmonary metastasis. The American journal of pathology, 2007, 170. 4: 1337-1347.

[PrimeSurface® Other application Publication List]

  1. ICHIOKA, Masayuki, et al. Dienogest, a synthetic progestin, down-regulates expression of CYP19A1 and inflammatory and neuroangiogenesis factors through progesterone receptor isoforms A and B in endometriotic cells. The Journal of steroid biochemistry and molecular biology, 2015, 147. 103-110.
  2. MORI, Taisuke, et al. Dienogest reduces HSD17β1 expression and activity in endometriosis. Journal of Endocrinology, 2015, 225. 2: 69-76.
  3. PARSONS, Matthew W, et al. Dectin-2 Regulates the Effector Phase of House Dust Mite–Elicited Pulmonary Inflammation Independently from Its Role in Sensitization. The Journal of Immunology, 2014, 192. 4: 1361-1371.
  4. BRESLIN, Susan and O’DRISCOLL, Lorraine Three-dimensional cell culture: the missing link in drug discovery. Drug Discovery Today, 2013, 18. 5: 240-249.
  5. BARRETT, Nora A, et al. Cysteinyl leukotriene 2 receptor on dendritic cells negatively regulates ligand-dependent allergic pulmonary inflammation. The Journal of Immunology, 2012, 189. 9: 4556-4565.
  6. MASUDA, Taisuke, et al. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomedical microdevices, 2012, 14. 6: 1085-1093.
  7. SOMA, Tsutomu, et al. Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation. Experimental dermatology, 2012, 21. 4: 307-309.
  8. YAMANAKA, Kaoruko, et al. Dienogest inhibits aromatase and cyclooxygenase-2 expression and prostaglandin E2 production in human endometriotic stromal cells in spheroid culture. Fertil Steril, 2012, 97. 2: 477-482.
  9. BRENNAN, Patrick J, et al. Invariant natural killer T cells recognize lipid self antigen induced by microbial danger signals. Nature immunology, 2011, 12. 12: 1202-1211.
  10. YOSHIIKE, Yuka and KITAOKA, Takuya Tailoring hybrid glyco-nanolayers composed of chitohexaose and cellohexaose for cell culture applications. Journal of Materials Chemistry, 2011, 21. 30: 11150-11158.
  11. MAEKAWA, Akiko, et al. GPR17 regulates immune pulmonary inflammation induced by house dust mites. The Journal of Immunology, 2010, 185. 3: 1846-1854.
  12. TAMADA, Atsushi, et al. Autonomous right-screw rotation of growth cone filopodia drives neurite turning. The Journal of Cell Biology, 2010, 188. 3: 429-441.
  13. IJIMA, Hiroyuki, et al. Composition of culture medium is more important than co-culture with hepatic non-parenchymal cells in albumin production activity of primary rat hepatocytes, and the effect was enhanced by hepatocytes spheroid culture in collagen gel. BIOCHEMICAL ENGINEERING JOURNAL, 2009, 45. 3: 226-231.
  14. ITO, Michiko and TAGUCHI, Tetsushi Enhanced insulin secretion of physically crosslinked pancreatic β-cells by using a poly (ethylene glycol) derivative with oleyl groups. Acta Biomater, 2009, 5. 8: 2945-2952.
  15. KATAOKA, M, et al. Detection of biomarker for periodontal disease using a microchip.2008,

Available Options

Catalog No.NameQty per CasePriceQuantity
#MS-90350ZPrimeSurface® 3D culture: Ultra-low Attachment 35 mm dish50$103.00
#MS-90600ZPrimeSurface® 3D culture: Ultra-low Attachment 60 mm dish100$552.00
#MS-90900ZPrimeSurface® 3D culture: Ultra-low Attachment 90 mm dish50$431.00
#MS-90240ZPrimeSurface® 3D culture: Ultra-low Attachment 24 well flat bottom plate10$138.00
#MS-9096UZPrimeSurface® 3D culture: Ultra-low Attachment Plates: 96 well, U bottom, Clear plates20$346.00
#MS-9096WZPrimeSurface® 3D culture: Ultra-low Attachment Plates: 96 well, U bottom, White plates20$460.00
#MS-9096VZPrimeSurface® 3D culture: Ultra-low Attachment Plates: 96 well, V bottom, Clear plates20$576.00
#MS-9384UZPrimeSurface® 3D culture: Ultra-low Attachment Plates: 384 well, U bottom, Clear plates20$576.00
#MS-9384WZPrimeSurface® 3D culture: Ultra-low Attachment Plates: 384 well, U bottom, White plates20$718.00

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