BlotGlyco® O-Glycan Sample Preparation Kit is a streamlined process for releasing and purifying O-glycans for detection by HPLC, LC-MS, MALDI-TOF MS.
- BlotGlyco® O-Glycan Sample Preparation Kit are used for O-glycan analysis in a broad range of biological samples such as purified glycoproteins and crude samples such as unpurified glycoproteins, serum, urine, cell and tissue lysates and plant extracts.
- The kit allows flexibility for choice of label* with detection methods including HPLC, LC-MS, MALDI-TOF MS or CE.
- BlotGlyco® beads are coated with dense hydrazide functional groups, which enable specific binding with aldehyde groups at the reducing end of O-glycan. The hydrazide groups on the beads form stable covalent bond with aldehyde; therefore, it allows the vigorous washing steps to eliminate peptide and other impurities.