Application using Sbio PrimeSurface® 96U Plates
Kenji Matsui, Shuichiro Yamanaka, Sandy Chen, Naoto Matsumoto, Keita Morimoto, Yoshitaka Kinoshita, Yuka Inage, Yatsumu Saito, Tsuyoshi Takamura, Toshinari Fujimoto, Susumu Tajiri, Kei Matsumoto, Eiji Kobayashi and Takashi Yokoo
Commun Biol 6, 1097 (2023). https://doi.org/10.1038/s42003-023-05484-9
Copyright © Authors 2023
This article is licensed under a Creative Commons Attribution 4.0 International License (CC BY).
Background
Organoids have garnered attention as tools for evaluating developmental and pathological models. Kidney organoids generated in vitro can mature by receiving blood supply from the host mouse after transplantation. However, the lack of connection to the urinary tract limits their long-term viability. Establishing aconnection between exogenous nephrons and the host urinary tract would extend their viability and enhance their clinical utility as a kidney model in which functionality and response to nephrotoxicity can be assessed.
【Fig.５】Long-term viability and excretion function of chimeric nephrons
Research Achievements
In this study, the group has developed the neonatal niche injection (NNI) method. This method proved to be highly approachable for generating chimeric nephrons by injecting renal progenitor cells (RPCs) and RPC spheroids produced by PrimeSurface® into neonatal mice. These chimeric nephrons with a connection to the host urinary tract exhibit maturation levels comparable to host nephrons, remain viable over an extended period, and are valuable for evaluating nephrotoxicity. Furthermore, hiPSC-derived progenitor cells also differentiate into nephrons using this method.
Use of PrimeSurface in this Study
- PrimeSurface® 96U plates were used for renal progenitor cell spheroid formation.
Suspended mouse nephron pellets of E14.5 and seeded to 2×105 cells/well.
Centrifuged the plate at 1000 rpm for 4 minutes and incubated overnight at 37°C, 5% CO2 incubator. Transplanted formed spheroids into NOG mice by NNI method. - PrimeSurface® 96V and U plates were used for renal progenitor cells differentiation of hiPSCs
Formed aggregates of hiPSCs on 96V plates and then transferred to U plates for differentiation induction by changing the medium.
