This article, "The Regulation Pathway of VEGF Gene Expression Is Different between 2D Cells and 3D Spheroids in Human Lung Cancer Cells" analyzes the VEGF gene expression pathway in 3D Spheroids of human lung cancer cells (PrimeSurface 96V MS-9096VZ) and compares it to 2D cells. highlighting the usefulness of 3D Spheroids for studying angiogenesis induction [...]
Interesting publication from National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo using ProteoSave, low bind tubes
"Increased cerebrospinal fluid complement C5 levels in major depressive disorder and schizophrenia" published in Biochemical and Biophysical Research Communications, Volume 497, Issue 2, 4 March 2018, Pages 683-688 mentions the use of ProteoSave 15 ml low bind tubes.
High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry
Check out this very interesting article published in SLAS Technol. 2017 Aug;22(4):454-465. doi: 10.1177/2211068216652846. Epub 2016 Jun 6 High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry
Culturing cells in three-dimensions (3D) has become a well established approach as it is more representative of the in vivo environment than traditional two-dimensional (2D) cultures. Allowing cells to interact with each other in a spheroid creates a micro-environment which mimics in vivo tissue and is a better model for examining the effect of drugs [...]
OZAWA, Masayuki (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports. 5: 1-16.
Madin-Darby canine kidney cell: MDCK T23 cells, anchorage independence growth of tumor cells, Immunoprecipitation and immunoblotting Flow cytometry Anoikis assay Fluorescence microscopy WST-1 cell pr liferation assay Soft agar colony assay Gene expression microarray and data analysis, Phase contrast micrograph Fluorescence microscopy, anchorage independence growth of tumor cells E-cadherin N-cadherin YAP/TAZ signaling, Doxorubicin (DOX), PrimeSurface
High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry.
Glioblastoma: U87MG, high-throughput screening by Image cytometry, Spheroid and Cell Viability Spheroid Size and Adherent Cell Confluence Measurement, Fluorescent Staining Celigo Image Cytometer, 3D tumor spheroid, spheroid size, spheroid viability, glioblastoma, high-throughput screening, image cytometry, Celigo, 17-AAG paclitaxel TMZ doxorubicin(DOX), MS-9384U