The plate surface was treated by the unique polymer to block non-specific binding of biological molecules. Without the blocking step, high sensitive immunoassay and hybridization assay can be constructed.

elisa-avidin-principle
1. Blocking steps and blocking reagents are not required

2. High capability of biotin immobilization:

blockingless-biotin

avidin-blockingless-biotin-immobilization

Comparison of biotin- HRP binding capacity on Avidin plate. The amount of bound biotin-HRP was measured by HRP activity. Avidin Plate (blocking-less type) showed higher capabilities of biotin-HRP than competitor products.

3. Very Low Background

blockingless-lowbackground

avidin-blockingless-verylowbackground

Comparison of non-specific binding. The amount of non-specific binding was examined using three HRP labeled proteins (BSA, mouse IgG, Streptavidin).

Avidin Plate (blocking-less type) showed the lowest background irrespective of proteins.

  • Immunoassays using biotin labeled antibody or other ligand
  • DNA/RNA hybridization assay using biotin labeled oligonucleotide

We also provide regular Blocking-less type ELISA plates (without avidin coated) which has good performance in blocking non-specific binding of biological molecules.