Publications

Co-culture system of human skin equivalents with mouse neural Spheroids

The article, “Co-culture system of human skin equivalents with mouse neural Spheroids”, introduces a co-culture system of human skin equivalents (HSEs) and dorsal root ganglion (DRG) neurons, using Spheroids (PrimeSurface 96U MS-9096UZ) of mouse DRG neurons with or without Schwann cells (SCs). Spheroids comprising DRG neurons and SCs demonstrated longer neurite extensions, and the model was suggested as a useful tool for studying neurite extension in human skin.

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Expansion culture of hair follicle stem cells through uniform aggregation in microwell array devices

The article, “Expansion culture of hair follicle stem cells through uniform aggregation in microwell array devices”, describes a novel approach for the quantitative expansion of hair follicle stem cells (HFSCs) while maintaining their hair induction capacity. Hairfollicle organoids were produced using low attachment plates (PrimeSurface 96U MS-9096UZ). By using a microwell array device and encapsulating HFSCs in Matrigel, researchers achieved a 20-fold increase in HFSC number in just two weeks, which may be beneficial for hair regeneration in the treatment of alopecia.

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Volumetric Compression by Heterogeneous Scaffold Embedding Promotes Cerebral Organoid Growth and Maturation

The article, “Volumetric Compression by Heterogeneous Scaffold Embedding Promotes Cerebral Organoid Growth and Maturation”, presents a new method for embedding cerebral organoids (PrimeSurface 96U MS-9096UZ) that mimics the mechanical environment of brain development. This method improves the maturation of organoids and enhances the expression of neuronal markers without hindering their growth.

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Biliary epithelial cell differentiation of bipotent human liver-derived organoids by 2D and 3D culture

The article, “Biliary epithelial cell differentiation of bipotent human liver-derived organoids by 2D and 3D culture”, discusses the differentiation potency of bipotent human liver-derived organoids (PrimeSurface 24F MS-90240Z) towards mature biliary epithelial cells (BECs). The study successfully demonstrates that bipotent hLOs can differentiate into mature BECs, both hepatocytes and BECs, making them a potential tool for various applications.

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ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma

The article, “ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma”, investigates alveolar soft part sarcoma (ASPS) and its prominent angiogenic activity, focusing on the role of the fusion transcription factor ASPSCR1::TFE3 in tumor development and angiogenesis. The study reveals that ASPSCR1::TFE3 is required for in vivo tumor development (using PrimeSurface 96U MS-9096UZ) through angiogenesis, as it influences super-enhancer activity and regulates critical targets involved in the construction of the ASPS vascular network.

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Novel Liposomal Formulation of Baicalein for the Treatment of Pancreatic Ductal Adenocarcinoma: Design, Characterization, and Evaluation

The article, written at the University of Wrocław, focuses on the development of a liposomal formulation containing the naturally-derived bioactive compound baicalein (BAI) for potential anti-pancreatic cancer therapy. The BAI-encapsulated liposomal suspension demonstrated high cytotoxic activity against pancreatic cancer cell lines, even spheroids (PrimeSurface 96U MS-9096UZ), while showing limited toxicity towards normal cells, making it a promising candidate for further exploration as an anticancer treatment for pancreatic ductal adenocarcinoma.

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Prophylactic administration of human amniotic fluid stem cells suppresses inflammation-induced preterm birth via macrophage polarization

The article, writen by researchers at th Keio University School of Medicine, investigates the therapeutic potential of human amniotic fluid stem cells (hAFSCs) for inflammation-induced preterm birth using a preterm mouse model of ascending infection. The study demonstrates that hAFSC (grown using PrimeSurface 96U (MS-9096UZ) administration significantly reduces inflammation-induced preterm birth, suppresses inflammation of the placenta and cervix, and switches peritoneal macrophages from an inflammatory to an anti-inflammatory type, suggesting hAFSCs’ effectiveness in treating and preventing inflammation-induced preterm birth.

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Targeting a check point receptor of human primary natural killer cells via CRISPR/Cas9 potentiates anti-tumor activity in allogeneic glioblastoma

The article describes the development of an immunotherapy strategy using genome-edited natural killer cells (NKCs) with TIGIT checkpoint receptor knockout to overcome the immunosuppressive tumor microenvironment in glioblastomas (GBM). The TIGIT knockout NKCs demonstrated enhanced anti-tumor activity against GBM cells and Spheroids, suggesting that this approach holds promise as a potential therapy for GBM. PrimeSurface 96well Vbottom plates (MS-9096VZ) were used for spheroid formation.

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Anticancer effect of a pyrrole‐imidazole polyamide‐triphenylphosphonium conjugate selectively targeting a common mitochondrial DNA cancer risk variant in cervical cancer cells

This article explores the efficacy of CCC-h1005, a TPP-conjugated pyrrole-imidazole polyamide, in targeting a common mtDNA cancer risk variant in cervical cancer cells. CCC-h1005 induced cell death, inhibited tumor growth, and holds promise as a potential treatment for cervical cancer and other cancers with high copies of the target variant.

PrimeSurface 96-well U bottom ultra low attachment plates (MS-9096UZ) were used for spheroid formation.

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GSK-3α/β and MEK inhibitors assist the microenvironment of tumor initiation

The article describes a method for converting human induced pluripotent stem cells (iPSCs) into cancer stem cells (CSCs) using a media containing conditioned medium (CM) from human pancreatic cancer cells supplemented with specific inhibitors (MEK inhibitor and GSK-3α/β inhibitor). The converted cells exhibited CSC characteristics and could be a valuable tool for modeling diseases and developing personalized cancer models to investigate tumor initiation and screen personalized therapies on CSCs.

PrimeSurface 24 ultra-low attachment plates (MS-90240Z) were used to generate spheroids using 1000 cells/well

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Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity

“Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity” The article highlights different subpopulations with different growth abilities and drug sensitivity in CRCs. It discusses intratumor heterogeneity and cellular plasticity in colorectal cancer organoids. The study identifies distinct subpopulations within clonal organoids, with some cells generating only small Spheroids (S-pattern) and others generating both small and large Spheroids (D-pattern) in response to extrinsic triggers, involving Notch signaling and Musashi-1. Targeting this transition from S-pattern to D-pattern could potentially be a therapeutic strategy to address drug resistance in colorectal cancer.

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  • PrimeSurface Spheroid Plates

Organosilica nanoparticles containing sodium borocaptate (BSH) provide new prospects for boron neutron capture therapy (BNCT): efficient cellular uptake and enhanced BNCT efficacy

A novel type of mesoporous silica-based nanoparticle (BSH-BPMO) containing covalently attached boron-10, a crucial component for boron neutron capture therapy (BNCT) that is conducted by Kyoto University. The nanoparticles efficiently enhance boron internalization in cancer cells, leading to improved BNCT efficacy compared to the commonly used drugs BSH and BPA in clinical trials. Note: spheroids were created using ultra low attachment plates PrimeSurface 96U (MS-9096UZ).

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https://pubs.rsc.org/en/content/articlelanding/2023/na/d2na00839d

  • PrimeSurface Spheroid Plates

Hypoxia inducible factor-1α promotes trichogenic gene expression in human dermal papilla cells

The article, published in Nature Scientific Reports by the Seoul National University College of Medicine, investigates the role of hypoxia-inducible factor-1α (HIF-1α) in hair follicle development using PrimeSurface 96U (MS-9096UZ) and regeneration in androgenetic alopecia patients. The study reveals that HIF-1α is suppressed in scalp tissues of these patients and demonstrates that activating HIF-1α can elevate the expression of trichogenic genes, suggesting its potential as a therapeutic target and tissue engineering approach for hair loss treatment.

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https://doi.org/10.1111/aji.13689

  • PrimeSurface Spheroid Plates

The Tpl2‐MEK pathway plays a critical role in Spheroid‐cultured endometriotic stromal cells

The article, published by Kyoto Prefectural University of Medicine “The Tpl2‐MEK pathway plays a critical role in Spheroid‐cultured endometriotic stromal cells” investigates the signaling pathway involved in cell growth and PGE2 production induced by TNF-α in endometriotic stromal cells that were cultured into spheroids using PrimeSurface 96U clear plate (MS-9096UZ), suggesting their potential as therapeutic targets for endometriosis.

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https://doi.org/10.1111/aji.13689

  • PrimeSurface Spheroid Plates

3D Spheroids of Human Lung Cancer Cells using S-Bio PrimeSurface Plates

This article, “The Regulation Pathway of VEGF Gene Expression Is Different between 2D Cells and 3D Spheroids in Human Lung Cancer Cells” analyzes  the VEGF gene expression pathway in 3D Spheroids of human lung cancer cells (PrimeSurface 96V MS-9096VZ) and compares it to 2D cells. highlighting the usefulness of 3D Spheroids for studying angiogenesis induction mechanisms.

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https://doi.org/10.1111/aji.13689

  • BioTek

Brightfield and Fluorescence Imaging using 3D PrimeSurface® Ultra-Low Attachment Microplates

Culturing cells in three-dimensions (3D) has become a well established approach as it is more representative of the in vivo environment than traditional two-dimensional (2D) cultures. Allowing cells to interact with each other in a spheroid creates a micro-environment which mimics in vivo tissue and is a better model for examining the effect of drugs in cancer. Developing uniform spheroids becomes especially important as it forms the basis for robust and reliable assays. S-BIO PrimeSurface® cultureware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly spheroid formation. The plates are pre-coated with a proprietary hydrophilic polymer that enables spontaneous spheroid formation of uniform size. PrimeSurface 96 and 384 ULA plates have good optical clarity making them highly suitable for brightfield and fluorescent imaging. Imaging technologies such as the BioTek Cytation™ 5 allows researchers to study not only spheroid proliferation through brightfield imaging, but also phenotypic events such as hypoxia, apoptosis, or necrosis induction through the use of fluorescent probes and fluorescence imaging. Incorporation of z-stacking and projection techniques in the Gen5™ Microplate Reader and Imager Software create in-focus images of spheroidal cells, allowing accurate, robust, and repeatable determination of the effect of test molecules or conditions. In this app note, we present data generated with BioTek Cytation 5 using PrimeSurface ULA plates to develop simple robust spheroid assays for brightfield and fluorescence imaging.

DOWNLOAD THE FULL APPLICATION NOTES: (pdf)
Biotek_Brightfield and Fluorescent S-BIO Imaging App_Note

OZAWA, Masayuki (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports. 5: 1-16.

Madin-Darby canine kidney cell: MDCK T23 cells, anchorage independence growth of tumor cells, Immunoprecipitation and immunoblotting Flow cytometry Anoikis assay Fluorescence microscopy WST-1 cell pr liferation assay Soft agar colony assay Gene expression microarray and data analysis, Phase contrast micrograph Fluorescence microscopy, anchorage independence growth of tumor cells E-cadherin N-cadherin YAP/TAZ signaling, Doxorubicin (DOX), PrimeSurface