Publications
3D Spheroids of Human Lung Cancer Cells using S-Bio PrimeSurface Plates
This article, “The Regulation Pathway of VEGF Gene Expression Is Different between 2D Cells and 3D Spheroids in Human Lung Cancer Cells” analyzes the VEGF gene expression pathway in 3D Spheroids of human lung cancer cells (PrimeSurface 96V MS-9096VZ) and compares it to 2D cells. highlighting the usefulness of 3D Spheroids for studying angiogenesis induction mechanisms.
Interesting publication from National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo using ProteoSave, low bind tubes
“Increased cerebrospinal fluid complement C5 levels in major depressive disorder and schizophrenia” published in Biochemical and Biophysical Research Communications, Volume 497, Issue 2, 4 March 2018, Pages 683-688 mentions the use of ProteoSave 15 ml low bind tubes.
High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry
Check out this very interesting article published in SLAS Technol. 2017 Aug;22(4):454-465. doi: 10.1177/2211068216652846. Epub 2016 Jun 6
Brightfield and Fluorescence Imaging using 3D PrimeSurface® Ultra-Low Attachment Microplates
Culturing cells in three-dimensions (3D) has become a well established approach as it is more representative of the in vivo environment than traditional two-dimensional (2D) cultures. Allowing cells to interact with each other in a spheroid creates a micro-environment which mimics in vivo tissue and is a better model for examining the effect of drugs in cancer. Developing uniform spheroids becomes especially important as it forms the basis for robust and reliable assays. S-BIO PrimeSurface® cultureware are ultra low attachment (ULA) dishes and plates that promote scaffold free, self assembly spheroid formation. The plates are pre-coated with a proprietary hydrophilic polymer that enables spontaneous spheroid formation of uniform size. PrimeSurface 96 and 384 ULA plates have good optical clarity making them highly suitable for brightfield and fluorescent imaging. Imaging technologies such as the BioTek Cytation™ 5 allows researchers to study not only spheroid proliferation through brightfield imaging, but also phenotypic events such as hypoxia, apoptosis, or necrosis induction through the use of fluorescent probes and fluorescence imaging. Incorporation of z-stacking and projection techniques in the Gen5™ Microplate Reader and Imager Software create in-focus images of spheroidal cells, allowing accurate, robust, and repeatable determination of the effect of test molecules or conditions. In this app note, we present data generated with BioTek Cytation 5 using PrimeSurface ULA plates to develop simple robust spheroid assays for brightfield and fluorescence imaging.
DOWNLOAD THE FULL APPLICATION NOTES: (pdf)
Biotek_Brightfield and Fluorescent S-BIO Imaging App_Note
OZAWA, Masayuki (2015). The N-cadherin cytoplasmic domain confers anchorage-independent growth and the loss of contact inhibition. Scientific Reports. 5: 1-16.
Madin-Darby canine kidney cell: MDCK T23 cells, anchorage independence growth of tumor cells, Immunoprecipitation and immunoblotting Flow cytometry Anoikis assay Fluorescence microscopy WST-1 cell pr liferation assay Soft agar colony assay Gene expression microarray and data analysis, Phase contrast micrograph Fluorescence microscopy, anchorage independence growth of tumor cells E-cadherin N-cadherin YAP/TAZ signaling, Doxorubicin (DOX), PrimeSurface
High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry.
Glioblastoma: U87MG, high-throughput screening by Image cytometry, Spheroid and Cell Viability Spheroid Size and Adherent Cell Confluence Measurement, Fluorescent Staining Celigo Image Cytometer, 3D tumor spheroid, spheroid size, spheroid viability, glioblastoma, high-throughput screening, image cytometry, Celigo, 17-AAG paclitaxel TMZ doxorubicin(DOX), MS-9384U